We also operate several light microscopes, including a Leica Thunder instrument, which allows to rapidly record fluorescent images at conventional light microscopy resolution (about 200nm at best), using the Thunder algorithm. This algorithm translates recorded images into a "Gauss-Blob Space", in which the image is decomposed into a superposition of Gaussian blobs, each at a specific X/Y position and of a certain diameter, brightness and color. The algorithm then allows to synthesize a new output image from that information, by only considering data from Gauss blobs of a certain diameter. The resulting images then give a much cleaner impression. This algorithm allows to reconstruct images from a thicker sample by only showing fluorophores in a certain depth of the sample (i.e., Gauss blob diameter). The effect is similar to that of a confocal light microscope, but is obtained much faster.
We also operate a Leica Laser Dissection light microscope, which allows to cut precisely shaped fractions from samples, which can later be used for further sample processing towards imaging by electron microscopy.