Mohamed Chami | LBEM

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Mohamed Chami

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Publications
-+

Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation

Botte, M., Ni, D., Schenck, S., Zimmermann, I., Chami, M., Bocquet, N., Egloff, P., Bucher, D., Trabuco, M., Cheng, R.K.Y., Brunner, J.D., Seeger, M.A., Stahlberg, H., Hennig, M.

Nature Communications 13(1), 1826 (2022)

29

Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog

Biou, V., Adaixo, R., Chami, M., Coureux. P., Laurent B., Ntsogo, Y., Cardoso de Amorim, G., Izadi-Pruneyre, N., Malosse, C., Chamot-Rooke, J., Stahlberg, H., Delepelaire, P.

Commun. Biol. 5(1), 355 (2022)

28

Cryo-EM structural studies of the agonist complexed human TRPV4 ion-channel reveals novel structural rearrangements resulting in an open-conformation

Botte, M., Ulrich, A.K.C., Adaixo, R., Gnutt, D., Brockmann, A., Bucher, D., Chami, M., Bocquet, N., Ebbinghaus-Kintscher, U., Puetter, V., Becker, A., Egner, U., Stahlberg, H., Hennig, M., Holton, S.J.

bioRxiv , 2020.10.13.334797 (2020)

27

Structural basis of Focal Adhesion Kinase activation on lipid membranes

Acebrón, I., Righetto, R., Schoenherr, C., de Buhr, S., Redondo, P., Culley, J., Rodríguez, C.F., Daday, C., Biyani, N., Llorca, O., Byron, A., Chami, M., Gräter. F., Boskovic J., Frame, M.C., Stahlberg, H., Lietha, D.

EMBO J. 39(e104743), (2020)

26

Self-assembly of a designed nucleoprotein architecture through multimodal interactions

Subramanian, R.H., Smith, S.J., Alberstein, R.G., Bailey, J.B., Zhang, L., Cardone, G., Suominen, L., Chami, M., Stahlberg, H., Baker, T.S., Tezcan, F.A.

ACS Central Science 4(11), 1578-1586 (2018)

25

Retrieving high-resolution information from disordered 2D crystals by single particle cryo-EM

Righetto, R., Biyani, N., Kowal, J., Chami, M., Stahlberg, H.

Nature Communications 10(1), 1722 (2019)

DOI: Nature Comm.

24

Structural basis for regulation of human acetyl-CoA carboxylase

Hunkeler, M., Hagmann, A., Stuttfeld, E., Chami, M., Guri, Y., Stahlberg, H., Maier, T.

Nature 558, 470-474 (2018)

23

Image processing techniques for high-resolution structure determination from badly ordered 2D crystals

Biyani, N., Scherer, S., Righetto, R., Kowal, J., Chami, M., Stahlberg, H.

Journal of Structural Biology 203(2), 120-134 (2018)

22

Miniaturized sample preparation for transmission electron microscopy

Schmidli, C., Rima, L., Arnold, S., Stohler, T., Syntychaki, A., Bieri, A., Albiez, S., Goldie, K., Chami, M., Stahlberg, H., Braun, T.

Journal of Visual Experiments (JOVE) (137), (2018)

21

Miniaturizing EM Sample Preparation: Opportunities, Challenges and "Visual Proteomics"

Arnold, S., Müller, S., Schmidli, C., Syntychaki, A., Rima, L., Chami, M., Stahlberg, H., Goldie, K., Braun, T.

Proteomics 18(5-6), e1700176 (2018)

20

High-resolution cryo-EM structure of the cyclic nucleotide-modulated potassium channel MloK1 in a lipid bilayer

Kowal, J., Biyani, N., Chami, M., Scherer, S., Rzepiela, A., Baumgartner, P., Upadhyay, V., Nimigean, C.N., Stahlberg, H.

Structure 26(1), 20-27 (2018)

19

Cryo-EM analysis of homodimeric full-length LRRK2 and LRRK1 protein complexes

Sejwal, K., Chami, M., Remigy, H., Vancraenenbroeck, R., Sibran, W., Sütterlin, R., Baumgartner, P., McLeod, R., Chartier-Harlin. M.-C., Baekelandt, V., Stahlberg, H., Taymans, J.-M.

Scientific Reports 7(1), 8667-8678 (2017)

18

Proteoliposomes - a system to study membrane proteins under buffer gradients by cryo-EM

Sejwal, K., Chami, M., Baumgartner, P., Kowal, J., Müller, S., Stahlberg, H.

Nanotechnology Reviews 6(1), 57-74 (2017)

17

Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor

Rheinberger, J., Hassaine, G., Chami, M., Stahlberg, H., Vogel, H., Li, X.

Micron 90, 19-24 (2017)

DOI: j.micron.2016.10.004

16

Biochemical and biophysical approaches to study the structure and function of the chloride channel (ClC) family of proteins

Abeyrathne, P., Chami, M., Stahlberg, H.

Biochimie 128-129, 154-162 (2016)

DOI: 10.1016/j.biochi.2016.08.008

15

Total sample conditioning and preparation of nanoliter volumes for electron microscopy

Arnold, S., Albiez, S., Opara, N., Chami, M., Schmidli, C., Bieri, A., Padeste, C., Stahlberg, H., Braun, T.

ACS Nano 10, 4981-4988 (2016)

DOI: 10.1021/acsnano.6b01328

14

The lipidome associated with the gamma-secretase complex is required for its integrity and activity.

Ayciriex, S., Gerber, H., Osuna, G.M., Chami, M., Stahlberg, H., Shevchenko, A., Fraering, P.C.

Biochemical Journal 473(3), 321-334 (2016)

DOI: 10.1042/BJ20150448

13

Glasslike Membrane Protein Diffusion in a Crowded Membrane.

Munguira I., Casuso I., Takahashi H., Rico F., Miyagi A., Chami, M., Scheuring S.

ACS Nano 10(2), 2584-90 (2016)

DOI: 10.1021/acsnano.5b07595

12

Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy

Sborgi, L., Ravotti, F., Dandey, V., Dick, M. S., Mazur, A., Reckel, S., Chami, M., Scherer, S., Huber, M., Bockmann, A., Egelman, E. H., Stahlberg, H., Broz, P., Meier, B. H., Hiller, S.

Proc Natl Acad Sci U S A 112(43), 13237-42 (2015)

11

2dx_automator: implementation of a semiautomatic high-throughput high-resolution cryo-electron crystallography pipeline

Scherer, S., Kowal, J., Chami, M., Dandey, V., Arheit, M., Ringler, P., Stahlberg, H.

J Struct Biol 186(2), 302-7 (2014)

10

Review: Cryo-electron microscopy of membrane proteins

Goldie, K., Abeyrathne, P., Kebbel, F., Chami, M., Ringler, P., Stahlberg, H.

In: Kuo J. (eds) Electron Microscopy. Methods in Molecular Biology (Methods and Protocols) Vol. 1117(Humana Press, Totowa, NJ, USA), 325-341 (2014)

9

Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1

Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P., Rangl, M., Scheuring, S., Schroder, G. F., Nimigean, C. M., Stahlberg, H.

Nat Commun 5, 3106 (2014)

8

Structure of the dodecameric Yersinia enterocolitica secretin YscC and its trypsin-resistant core

Kowal, J., Chami, M., Ringler, P., Müller, S., Kudryashev, M., Castano-Diez, D., Amstutz, M., Cornelis, G. R., Stahlberg, H., Engel, A.

Structure 21(12), 2152-61 (2013)

7

Molecular assembly of the aerolysin pore reveals a swirling membrane-insertion mechanism

Degiacomi, M. T., Iacovache, I., Pernot, L., Chami, M., Kudryashev, M., Stahlberg, H., van der Goot, F. G., Dal Peraro, M.

Nat Chem Biol 9(10), 623-9 (2013)

6

Analysis of 2-D crystals of membrane proteins by electron microscopy

Abeyrathne, P., Arheit, M., Kebbel, F., Castano-Diez, D., Goldie, K., Chami, M., Relault, L., Kühlbrandt, W., Stahlberg, H.

Comprehensive Biophysics 1, 277-310 (2012)

DOI: 10.1016/B978-0-12-374920-8.00119-3

5

Characterization of the motion of membrane proteins using high-speed atomic force microscopy

Casuso, I., Khao, J., Chami, M., Paul-Gilloteaux, P., Husain, M., Duneau, J. P., Stahlberg, H., Sturgis, J. N., Scheuring, S.

Nat Nanotechnol 7(8), 525-9 (2012)

4

Preparation of 2D crystals of membrane proteins for high-resolution electron crystallography data collection

Abeyrathne, P., Chami, M., Pantelic, R., Goldie, K., Stahlberg, H.

Method Enzymol 481, 25-43 (2010)

3

Double hexameric ring assembly of the type III protein translocase ATPase HrcN

Müller, S., Pozidis, C., Stone, R., Meesters, C., Chami, M., Engel, A., Economou, A., Stahlberg, H.

Mol Microbiol 61(1), 119-25 (2006)

2

Charting and unzipping the surface layer of Corynebacterium glutamicum with the atomic force microscope

Scheuring, S., Stahlberg, H., Chami, M., Houssin, C., Rigaud, J. L., Engel, A.

Mol Microbiol 44(3), 675-84 (2002)

1

2009-2020, Facility Head

+41 61 387 32 24

C-CINA, Biozentrum, Uni Basel, Mattenstrasse 26, Room P30, CH-4058 Basel, Switzerland

Mohamed.Chami@unibas.ch Personal Web Page